Koprov

Instance: piefed.social
Joined: 2 months ago
Posts: 0
Comments: 11

Posts and Comments by Koprov

That really depends on your budget.

What you need is a plasmid (a peace of DNA) with all of the machinery needed for cobalamin production on it. You can do in-silico modeling to get the plasmid sequence but then you will need to make it. In order to make a plasmid you can either do all the wetwork yourself, which requires a lot of equipment and reagents or you can pay a company to synthesize do it for you.

Once you have a plasmid the rest if pretty straightforward: get the plasmid into bacteria (can be done with ice and calcium salts), get the bacteria into a container with sterile media (a fancy chicken broth with salts) and let them rip :)

Finally, you will need to isolate your cobalamin form the used media, but you’ll need to look into that, I haven’t done it before (filtration and fractioning most likely) ;)

If you need specific guidance on the process and materials to do it at home then look into biohacking and DIY Science communities, maybe there is even a physical one somewhere nearby if you are lucky! ;)

Good luck and take care!


"You seem like someone who would enjoy Dubai" is an insult for a reason



Very specific subset of people, white catholic men to be exact. Also, he’s a nut who believes in conspiracy theories.


That’s insanity! It’s literally building a pile of TNT to sit upon and than handing everyone a match “for safety”!



Before you go, why not take one of them with you? Just a thought…



How is the Uyghur situation lately? Still chinamaxxing in their concentration camps? Fucking whitewashing…


All part of a plan called Seven Mountains Mandate: https://en.wikipedia.org/wiki/Seven_Mountain_Mandate

They know they f’ed up badly and this is their only chance for a long while, so they are going all in.


Figures that those ICE boys would also be spawn campers 😡


Posts by Koprov

Comments by Koprov

That really depends on your budget.

What you need is a plasmid (a peace of DNA) with all of the machinery needed for cobalamin production on it. You can do in-silico modeling to get the plasmid sequence but then you will need to make it. In order to make a plasmid you can either do all the wetwork yourself, which requires a lot of equipment and reagents or you can pay a company to synthesize do it for you.

Once you have a plasmid the rest if pretty straightforward: get the plasmid into bacteria (can be done with ice and calcium salts), get the bacteria into a container with sterile media (a fancy chicken broth with salts) and let them rip :)

Finally, you will need to isolate your cobalamin form the used media, but you’ll need to look into that, I haven’t done it before (filtration and fractioning most likely) ;)

If you need specific guidance on the process and materials to do it at home then look into biohacking and DIY Science communities, maybe there is even a physical one somewhere nearby if you are lucky! ;)

Good luck and take care!


"You seem like someone who would enjoy Dubai" is an insult for a reason



Very specific subset of people, white catholic men to be exact. Also, he’s a nut who believes in conspiracy theories.


That’s insanity! It’s literally building a pile of TNT to sit upon and than handing everyone a match “for safety”!



Before you go, why not take one of them with you? Just a thought…



How is the Uyghur situation lately? Still chinamaxxing in their concentration camps? Fucking whitewashing…


All part of a plan called Seven Mountains Mandate: https://en.wikipedia.org/wiki/Seven_Mountain_Mandate

They know they f’ed up badly and this is their only chance for a long while, so they are going all in.


Figures that those ICE boys would also be spawn campers 😡